Akademik Veri Yönetim Sistemi
INSAC International Researches Congress on Health and Life Sciences , Konya, Türkiye, 18 - 19 Mart 2023, ss.1-2
Abstract: Mesenchymal stem cells (MSCs) are of great importance in many
medical fields due to their abundance and ease of isolation from various sources,
as well as their allogeneic, immunomodulatory, anti-inflammatory, angiogenic,
and anti-apoptotic properties. Adipose tissue-derived MSCs (ADMSCs) have
become increasingly popular for therapeutic purposes in recent years due to their
ease of isolation, high multipotency, and safe use. However, conventional
polystyrene surface culture techniques have been shown to reduce the
immunomodulatory abilities of ADMSCs. In order to address this problem, studies
have been conducted using the HAVDI pentapeptide sequence, located in the first
extracellular part of N-cadherin and involved in cell-cell interactions, to establish
N-cadherin and improve ADMSC proliferation and regenerative capacity.
However, the immunomodulatory abilities of this technique have not yet been
investigated. In this study, experimental groups were formed using normal plastic
surfaces, HAVDI and Scrambled HAVDI sequences, and their effects on immune
response were investigated in 2D and 3D cell culture environments. RNA and
supernatants were collected from ADMSCs after 18 hours of stimulation with 0.1
µg/ml LPS. Results showed that the amount of IL-6 gene and protein increased in
cell cultures when the plates were coated with the HAVDI peptide amphiphile,
indicating positive effects of the HAVDI peptide sequence on the
immunomodulatory response of ADMSCs. In conclusion, the HAVDI peptide
sequence can be considered a strategy to enhance the yield of MSCs, which are
widely used in clinics. This study demonstrated the positive effects of the HAVDI
peptide sequence on the immunomodulatory abilities of ADMSCs, which may have
implications for the future development of MSC-based therapies.