HAVDI Bioactive Sequence Modulates Immune Responses Of Adipose Derived Mesenchymal Stem Cells In 2D and 3D Cell Culture System

Yüregir Y., Kaçaroğlu D., Yaylacı S.

INSAC International Researches Congress on Health and Life Sciences , Konya, Turkey, 18 - 19 March 2023, pp.1-2

  • Publication Type: Conference Paper / Summary Text
  • City: Konya
  • Country: Turkey
  • Page Numbers: pp.1-2
  • Lokman Hekim University Affiliated: Yes


Abstract: Mesenchymal stem cells (MSCs) are of great importance in many

medical fields due to their abundance and ease of isolation from various sources,

as well as their allogeneic, immunomodulatory, anti-inflammatory, angiogenic,

and anti-apoptotic properties. Adipose tissue-derived MSCs (ADMSCs) have

become increasingly popular for therapeutic purposes in recent years due to their

ease of isolation, high multipotency, and safe use. However, conventional

polystyrene surface culture techniques have been shown to reduce the

immunomodulatory abilities of ADMSCs. In order to address this problem, studies

have been conducted using the HAVDI pentapeptide sequence, located in the first

extracellular part of N-cadherin and involved in cell-cell interactions, to establish

N-cadherin and improve ADMSC proliferation and regenerative capacity.

However, the immunomodulatory abilities of this technique have not yet been

investigated. In this study, experimental groups were formed using normal plastic

surfaces, HAVDI and Scrambled HAVDI sequences, and their effects on immune

response were investigated in 2D and 3D cell culture environments. RNA and

supernatants were collected from ADMSCs after 18 hours of stimulation with 0.1

µg/ml LPS. Results showed that the amount of IL-6 gene and protein increased in

cell cultures when the plates were coated with the HAVDI peptide amphiphile,

indicating positive effects of the HAVDI peptide sequence on the

immunomodulatory response of ADMSCs. In conclusion, the HAVDI peptide

sequence can be considered a strategy to enhance the yield of MSCs, which are

widely used in clinics. This study demonstrated the positive effects of the HAVDI

peptide sequence on the immunomodulatory abilities of ADMSCs, which may have

implications for the future development of MSC-based therapies.