High cholesterol diet leads to oxidant load and peroxidation in the rabbit kidney tissue

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Ozturk B., ÖZER Ö. F., Durak Z., BİLLUR D., Kizil Ş., DURAK İ., ...Daha Fazla

Bratislava Medical Journal, cilt.117, sa.4, ss.235-241, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 117 Sayı: 4
  • Basım Tarihi: 2016
  • Doi Numarası: 10.4149/bll_2016_045
  • Dergi Adı: Bratislava Medical Journal
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.235-241
  • Anahtar Kelimeler: cholesterol, oxidant / antioxidant system, kidney, rabbit, LOW-DENSITY-LIPOPROTEIN, OXIDATIVE STRESS, LIPID-PEROXIDATION, NITRIC-OXIDE, RENAL-FUNCTION, GLOMERULAR ATHEROSCLEROSIS, TUBULOINTERSTITIAL DISEASE, GLUTATHIONE-PEROXIDASE, INTERSTITIAL FIBROSIS, HEMODIALYSIS-PATIENTS
  • Lokman Hekim Üniversitesi Adresli: Hayır

Özet

OBJECTIVES: The aim of this study was to investigate possible effects of high cholesterol diet on oxidant/antioxidant status in rabbit kidney tissues. BACKGROUND: Although a number of experimental animal models have suggested that hyperlipidemia is associated with progressive kidney failure data remain sparse on the role of dietary cholesterol intake on kidney disease. METHODS: Twelve male New Zealand albino rabbits were randomly divided into two groups (control and cholesterol). Both groups were fed on a standard laboratory diet. Animals in the cholesterol group additionally received cholesterol (1 g/kg/day), orally. The study period was 12 weeks. Activities of catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), nitric oxide synthase (NOS), xanthine oxidase (XO), paraoxonase (PON), adenosine deaminase (ADA) enzymes and levels of malondialdehyde (MDA) and nitric oxide (NO) were measured in kidney tissue samples. Histological examination of the kidney tissue samples was also done. RESULTS: SOD, GSH-Px and XO enzyme activities were found to be decreased and NOS and PON activities increased significantly in cholesterol group compared to controls. As an indication of oxidation, MDA levels were found to be increased in cholesterol group. Histological examination revealed some derangements in the kidney tissue. CONCLUSION: High cholesterol diet creates oxidant load and causes peroxidation, which in turn, leads derangements in the rabbit kidney tissue.