5th International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2022), Ankara, Turkey, 23 November 2022 - 25 January 2023, vol.1, no.0, pp.103
Fluoroquinolones are the main antibacterial drugs that are used against Gram-positive, Gram-negative, and atypical bacteria. They are divided into four generations. Monitoring concentrations of antibacterial agents provides effective therapy and prevents the increase of bacterial resistance to antibiotics. Also, it will give preservative treatment approach against the probably toxicological effects. The aim of the study is finding a new, easy method that allow the separation and simultaneous determination of four fluoroquinolones; ciprofloxacin, ofloxacin, enrofloxacin and moxifloxacin. The separation of fluoroquinolones studies in aqueous media are tried in many methods in which samples placed as single or their binary mixtures. In our study we aimed to find the most effective separation technique for mentioned above four fluoroquinolones. Different acidic and basic mobile phases, buffer solutions and elution types were tried. The most effective and selective method was performed on XTerra, C18 (100 x 4.6 mm, particle size 3.5 m) analytical column and methanol : borate buffer (pH=9.1, 100 mM) (50:50, v/v) containing mobile phase at 0.6 mL/min of flow rate. Generally, fluoroquinolones were determined by using HPLC-Fluorescence detector. In our study detection was realized by DAD detector at 280 nm. Any interference peaks were not observed and obtained good resolution. In the study, separation, and simultaneous determination of ciprofloxacin, ofloxacin, enrofloxacin and moxifloxacin separation were realized. The calibration curves were linear over the concentration ranges of 0.25-10 g/ml for studied quinolones. Validation studies of the developed method were also studied for these quinolones. This developed method describes a simple, sensitive, and practical HPLC-DAD method which permits to simultaneous determination of ciprofloxacin, ofloxacin, enrofloxacin and moxifloxacin in a mixture. We think that the method can also be applied in biological fluids.
Keywords: Fluoroquinolones, HPLC-DAD, ciprofloxacin, ofloxacin, enrofloxacin, moxifloxacin