Method validation of tandem mass spectrometry for 25-hydroxyvitamin D3 and comparison of this method with other methods 25-OH-vitamin D hormon için tandem kütle spektrometrede yöntem geçerli kılma çalışması ve bu yöntemin farklı yöntemlerle karşılaştırılması.

Sahillioǧlu B., Serdar M. A., Erkal N., Erden G., BAKIR F., Yildirimkaya M. M., ...Daha Fazla

Turkish Journal of Biochemistry, cilt.36, sa.1, ss.73-79, 2011 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 36 Sayı: 1
  • Basım Tarihi: 2011
  • Dergi Adı: Turkish Journal of Biochemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.73-79
  • Anahtar Kelimeler: Method comparison, Tandem mass spectrometry
  • Lokman Hekim Üniversitesi Adresli: Hayır

Özet

Objective: Measurement of circulating 25-hydroxyvitamin D3 is accepted as the clinical indicator of vitamin D status. It is very important of valid measurement of 25-hy-droxyvitamin D3 as clinical. Therefore there is need a standardization for measurement. Methods except tandem mass spectrometry (LC-MS/MS) measure total vitamin D and are less sensitive. LC-MS/MS is gold standard method for measuring vitamin D values. On this account we have invented a new method and evaluated this method to measure the imprecision and the accuracy and compared it with other systems. Materials and Methods: We used isotope-dilution LC-MS/MS (ID-LC-MS/MS) for determination of D3 with a stable-isotope-labeled internal standard. The values we obtained by ID-LC-MS/MS compared with HPLC, RIA and chemiluminescence methods. Results: The interassay CVs for 25-hydroxyvitamin D3 were 5.3%, 5.5% and 5.6% at 6.5, 24 and 43 ng/mL, respectively. The detection limit of the present method was <3.75 ng/ mL for 25-hydroxyvitamin D3. Total run time was 4 min. Method comparison between HPLC and LC-MS/MS showed a good correlation: y = 4.3822 + 1.0124 × (R2 = 0.96). Conclusions: We conclude that methods based on LC-MS/MS should be preferred in routine clinical work. We got the best results with HPLC according to the gold standard method, LC-MS/MS. © TurkJBiochem.com.