Background: Quinolones are an important group of synthetic antibiotics with bactericidal action. The antibacterial activity of these compounds has been shown to result from selective inhibition of bacterial DNA synthesis. It is a preferable option in clinical use because of its efficacy, reliability and wide antibacterial spectrum. The identification and quantification of quinolones in various biological tissues and fluids are needed. Methods: Numerous techniques have been developed for their analysis of biological materials and pharmaceutical dosage forms. In this review, the chromatographic methods published in the last ten years for the analysis of quinolones, using for the treatment of an infection in a human, were reviewed. Results: The analysis methods of quinolones as single or simultaneous in biological materials were summarized according to sample preparation, chromatographic conditions, validation parameters and applicability of the methods. The pH effect on the chromatographic behaviors of quinolones was discussed. Moreover, multivariate analysis was performed for the summarized published methods based on the method parameters. Conclusion: Many rapid and efficient separation methods have been developed for individual or simultaneous determination of quinolone. In this study, a comprehensive review is presented for the analysis of quinolone developed in the last decade.