Evaluation of preventive effect of quercetin on doxorubicin-induced nephrotoxic rat model by [99mTc]Tc-DMSA renal cortical scintigraphy and biochemical methods


Köroǧlu R., GÜL S. S., Aygun H.

Iranian Journal of Nuclear Medicine, vol.31, no.2, pp.112-118, 2023 (ESCI) identifier

  • Publication Type: Article / Article
  • Volume: 31 Issue: 2
  • Publication Date: 2023
  • Doi Number: 10.22034/irjnm.2023.129042.1536
  • Journal Name: Iranian Journal of Nuclear Medicine
  • Journal Indexes: Emerging Sources Citation Index (ESCI), Scopus, Academic Search Premier
  • Page Numbers: pp.112-118
  • Keywords: Doxorubicin, Kidney, Nephrotoxicity, Quercetin, [99mTc]Tc-DMSA
  • Lokman Hekim University Affiliated: No

Abstract

Published Online: 24 April 2023 Introduction: The purpose of this study was to evaluate possible preventive role of quercetin on doxorubicin (DOX) induced kidney toxicity using Tc-99m Dimercaptosuccinic Acid ([99mTc]Tc-DMSA) renal cortical scintigraphy and biochemical approaches. Methods: 28 Male wistar rats were separated into four groups. First group was intraperitoneally (i.p.) injected saline and regarded as the control group; second one was received 18 mg/kg/i.p doxorubicin for three days at a 24 h interval; the third and last group received 10 mg/kg and 100 mg/kg quercetin for 21 days and for the last 3 days doxorubicin and quercetin were administrated together at the same time. On the 22nd day of the experiment, [99mTc]Tc-DMSA renal cortical scintigraphy and biochemical parameters were measured. Results: DOX administration significantly increased blood urea nitrogen (845%) and creatinine (702%) levels in serum; nitric oxide (158%), plasma tumor necrosis factor-alpha (233%) and interleukin-6 (191%) levels in kidney tissue, and also reduced [99mTc]Tc-DMSA uptake by 29% in the kidneys as well. Pre-treatment with quercetin mitigated such alterations in all mentioned parameters. Conclusion: All data indicate that oxidative stress and inflammatory processes are involved in DOX-induced nephrotoxicity, which might be decreased by quercetin. In addition, [99mTc]Tc-DMSA scintigraphic may be a good method for demonstrating doxorobucin-induced renal injury.