Akademik Veri Yönetim Sistemi
7th International Eurasian Conference on Biological and Chemical Sciences, Ankara, Türkiye, 2 - 04 Ekim 2024, ss.111
The use of mesenchymal stem cells(MSCs) in clinical applications is
increasing due to their regenerative properties. It is known that
cadherin-based biomaterials enhance the adhesion of MSCs, improve their
paracrine effects, induce proliferation, increase anti-apoptotic effects, and
reduce fibrosis. N-cadherin biomimetic hydrogel may regulate the effects of
MSCs on endothelial cells by altering the secretion of specific growth factors,
but this effect has not yet been clearly defined. The aim of this study is to
investigate the effects of conditioned medium obtained from a 3D N-cadherin
mimetic environment on the viability and migration of endothelial cells. The
observed effects are also compared with 2D classical culture and 3D spheroid
culture. In this study, the purity and characterization of the synthesized
peptides were demonstrated by HPLC and Mass Spectrometry analyses. The
experimental groups were designed as 2D classical culture, 3D spheroid culture,
3D N-cadherin mimetic hydrogel (at a concentration of 1 mM), and 3D hydrogel
containing scram sequence peptides. To evaluate paracrine effects, the impact
of conditioned media obtained from 48-hour culture on the proliferation and
migration of Human Umbilical Vein Endothelial Cells (HUVECs) was investigated
using MTT viability analysis, calcein staining, and wound healing methods. We reported
that MSCs cultured in N-cadherin hydrogel secreted more PDGF-BB, FGF-2, and
VEGF-A growth factors compared to the 2D classical culture media, as
demonstrated by the ELISA method. Conditioned media obtained from the
N-cadherin hydrogel were demonsrated to increase the viability of endothelial
cells at 1:2 and 1:5 dilutions and to have a higher migration potential
compared to other groups. N-cadherin biomimetic peptide hydrogel may be
considered a more effective culturing strategy than the 2D classical culture to
enhance the paracrine effects of MSCs and their effects on endothelial cells.