Preparation and in vitro evaluation of bFGF-loaded chitosan nanoparticles


Cetin M., AKTAŞ Y., VURAL İ., ÇAPAN Y. , DOĞAN A., DUMAN M., ...More

Drug Delivery, vol.14, no.8, pp.525-529, 2007 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 14 Issue: 8
  • Publication Date: 2007
  • Doi Number: 10.1080/10717540701606483
  • Title of Journal : Drug Delivery
  • Page Numbers: pp.525-529
  • Keywords: bFGF, chitosan, nanoparticles, SDS-PAGE, TPP, FIBROBLAST-GROWTH-FACTOR, BLOOD-BRAIN-BARRIER, DELIVERY, MICROSPHERES, HYDROGEL, PROTECTION, PARTICLES, ISCHEMIA, FACTOR-2, TISSUE

Abstract

The objective of our study was to prepare and characterize basic fibroblast growth factor (bFGF)-loaded nanoparticles. Protein-loaded chitosan nanoparticles were obtained by ionotropic gelation process based on the interaction between chitosan and tripolyphosphate (TPP). The protein-loading capacity and encapsulation efficiency were 0.021% and 27.388%, respectively. The bFGF-loaded nanoparticles have a mean diameter of 424 nm, a narrow size distribution, spherical shape and positive surface charges. In vitro release showed that the extent of release was 68% at 24 hr. The protein integrity was investigated by SDS-PAGE analysis that confirmed protein integrity was not affected by the encapsulation procedure and release conditions. Copyright © Informa Healthcare USA, Inc.