Akademik Veri Yönetim Sistemi
Extracellular Vesicles Conference 2024, Ankara, Türkiye, 20 - 21 Eylül 2024, ss.96
Mesenchymal stem cells(MSCs) and their secreted exosomes have recently been explored as therapeutic candidate in targeted cancer therapy approaches. In response to the signals received from the environment, MSCs regulate the microenvironment by secreting cytokines, chemokines, growth factors, enzymes and exosomes. MSCs are also an essential modulator of the microenvironment in tumours consisting of a high amount of stroma. In this study, lung, pancreas and liver cancer cell lines were used. In this study, the viability and proliferation capacity of exosomes derived from Wharton Jelly(WJ)-MSCs on A549, Panc-1 and HepG2 cells were assessed. After 20 million exosomes were applied to the cells, cell viability was measured by MTT assay and proliferation was analysed by calcein staining at 48 and 96 hours. At the end of 96th hour, 62% increase in A549 cells, 95% increase in Panc-1 cells and 67% increase in HepG2 cells were detected in the control group. In the group which 20 million exosomes were applied, 36% increase in A549 cells, 43% increase in Panc-1 cells and 31% increase in HepG2 cells were observed. At 96 hour calcein staining, the fluorescence intensity of the control group (Excitation: 488 nm /Emission: 520 nm) was accepted as 100%. The fluorescence intensity of 20 million exosomes was calculated as; a549; 61%, Panc-1; 64% and HepG2; 68%. Preliminary findings indicate that WJ MSC-derived exosomes do not boost the viability and proliferation of cancer cells when applied to 5000 (per well) cancer cells 20 million. WJ-MSC derived exosomes might have an impact on in vitro cell proliferation in tumours with a high stroma content and other antitumour effects on tumour cells need to be explored.