The effects of di(2-ethylhexyl)phthalate on rat liver in relation to selenium status

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ERKEKOĞLU Ü. P., ZEYBEK N. D., Giray B., Rachidi W., Kizilgün M., Hininger-Favier I., ...Daha Fazla

International Journal of Experimental Pathology, cilt.95, sa.1, ss.64-77, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 95 Sayı: 1
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1111/iep.12059
  • Dergi Adı: International Journal of Experimental Pathology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.64-77
  • Anahtar Kelimeler: antioxidant enzymes, di(ethylhexyl)phthalate, liver, oxidative stress, selenium deficiency, selenium supplementation, OXIDATIVE STRESS, PEROXISOME PROLIFERATION, GLUTATHIONE-PEROXIDASE, NONGENOTOXIC CARCINOGENESIS, HEPATOCELLULAR-CARCINOMA, MAMMALIAN THIOREDOXIN, ANTIOXIDANT ENZYMES, SPECIES-DIFFERENCES, DIETARY SELENIUM, PHTHALATE DEHP
  • Lokman Hekim Üniversitesi Adresli: Hayır

Özet

This study was performed to determine the hepatotoxicity of di(2-ethylhexyl)phthalate (DEHP) in relation to selenium status. In 3-week-old Sprague-Dawley rats, selenium deficiency was induced by a ≤0.05 selenium mg/kg. A selenium supplementation group was given 1 mg selenium/kg diet for 5 weeks. Di(2-ethylhexyl)phthalate-treated groups received 1000 mg/kg dose by gavage during the last 10 days of the experiment. Histopathology, peroxisome proliferation, catalase (CAT) immunoreactivity and activity and apoptosis were assessed. Activities of antioxidant selenoenzymes [glutathione peroxidase 1 (GPx1), glutathione peroxidase 4 (GPx4), thioredoxin reductase (TrxR1)], superoxide dismutase (SOD), and glutathione S-transferase (GST); aminotransferase, total glutathione (tGSH), and lipid peroxidation (LP) levels were measured. Di(2-ethylhexyl)phthalate caused cellular disorganization while necrosis and inflammatory cell infiltration were observed in Se-deficient DEHP group (DEHP/SeD). Catalase activity and immunoreactivity were increased in all DEHP-treated groups. Glutathione peroxidase 1 and GPx4 activities decreased significantly in DEHP and DEHP/SeD groups, while GST activities decreased in all DEHP-exposed groups. Thioredoxin reductase activity increased in DEHP and DEHP/SeS, while total SOD activities increased in all DEHP-treated groups. Lipid peroxidation levels increased significantly in SeD (26%), DEHP (38%) and DEHP/SeD (71%) groups. Selenium supplementation partially ameliorated DEHP-induced hepatotoxicity; while in DEHP/SeD group, drastic changes in hepatic histopathology and oxidative stress parameters were observed. International Journal of Experimental Pathology © 2014 International Journal of Experimental Pathology.