Development of tumor-specific liposomes containing quantum dots-photosensitizer conjugate used for radiotherapy


Karabuga M., ERDOĞAN S., TİMUR S. S., VURAL İ., ÇALAMAK S., ULUBAYRAM K.

Journal of Liposome Research, vol.32, no.4, pp.396-404, 2022 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 32 Issue: 4
  • Publication Date: 2022
  • Doi Number: 10.1080/08982104.2022.2087082
  • Journal Name: Journal of Liposome Research
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, EMBASE, MEDLINE
  • Page Numbers: pp.396-404
  • Keywords: chlorine-e6, Liposome, quantum dot, radiosensitizer, radiotherapy
  • Lokman Hekim University Affiliated: Yes

Abstract

© 2022 Informa UK Limited, trading as Taylor & Francis Group.This study aims to develop a multifunctional liposomal radiosensitizer to destroy more tumor cells by using lower radiation doses compared to clinically used 6 MV X-ray doses. To achieve this aim, first Chlorine-e6 (Ce6) was covalently bound to functional groups of outer surfaces of quantum dots (QDs) through EDC/NHS reactions. Then, QDs-Ce6 conjugate loaded, nanosized, PEG-coated, and tumor-specific folic acid-modified immunoliposome dispersions were prepared by film method. Enhanced anti-proliferation activity of free and liposomal conjugate against 4T1 (murine breast cancer) cell lines was investigated at different X-ray doses (5, 10, 15, and 20 Gy). As a result, the best radiosensitizer effect was observed at a 5 Gy X-ray dose and it was found that following the X-ray irradiation, immunoliposome dispersions containing QDs-Ce6 conjugate killed 26.8 ± 1.7% more cancer cells than radiation alone.