Objective: To evaluate the effects of ghrelin, leptin and lipid levels on growth status of healthy infants fed with breastmilk (BF) and formula (FF). Material and Metods: This study was performed in 46 healthy infants. The study group was subdivided into two subgroups according to nutrition status as breastfed and formula infants. These infants were evaluated at two different times (mean of the second and fifth months, respectively). The ghrelin, leptin, triglyceride and total cholesterol levels of infants were studied at the first visit. Results: Plasma total ghrelin concentrations were significantly higher in BF group than FF group(p<0.05). Although the serum leptin level was not statisticaly significant, it was higher in BF group. In BF group, positive correlations were found between total ghrelin concentrations and mid-arm circumference and triceps skinfold thickness (TST) at first visit. Serum leptin levels were positively correlated to BMI at both visits (p<0.05) and TST at second visit (p<0.01). In the FF group, positive correlations were found between total ghrelin levels and head circumferences at first visit and body weight at second visit (p<0.05). One breastfeeding period was longer, feeding frequency and defecation frequency were higher in BF group than FM group (p<0.01, p<0.01, p<0.05; respectively). Conclusion: High ghrelin and leptin levels in BF group suggests that these hormones pass to the baby with breast milk, high ghrelin levels are effective on a more rapid weight gain in the first few months and have long term effects on energy metabolism. The lower ghrelin and leptin may be associated with contents of FF or type of bottle feeding in FF group. © 2011 Düzce Medical Journal.