Akademik Veri Yönetim Sistemi
7th International Symposium on Medicinal and Aromatic Plants (SIPAM-7), Madaniyin, Tunus, 11 - 13 Nisan 2025, ss.183-184, (Özet Bildiri)
Wound healing is a complex biological process that involves cell proliferation and migration,
particularly of dermal fibroblasts. Natural products have gained increasing attention as potential
agents to support and heal this process. In this study, we investigated the proliferative and
wound-healing effects of the cultured sample of Cynara scolymus L. (artichoke, Asteraceae)
leaf and stem extracts on human dermal fibroblast cells. Artichoke leaf and stem extracts were
prepared and applied to cultured human dermal fibroblast cells at concentrations ranging from
0.015 to 1 mg/mL. Cell viability was assessed using the MTT assay at 24 and 48 hours. In order
to evaluate the wound-healing potential, an in vitro scratch assay was performed and wound
closure was monitored at 0, 24, and 48 hours. The leaf extract demonstrated a concentration-
dependent effect on fibroblast proliferation and migration. At 0.031 and 0.062 mg/mL, the leaf extract significantly increased cell viability and promoted wound closure, indicating enhanced
proliferative and migratory activity. However, at concentrations of 0.250 mg/mL and above, a
marked reduction in both cell viability and wound closure capacity was observed. The stem
extract also exhibited moderate proliferative and wound-healing effects at lower concentrations
(0.015–0.062 mg/mL). Notably, the stem extract at concentration of 0.125 mg/mL induced
cytotoxic effects, characterized by decreased viability and observable morphological alterations
in fibroblast cells, suggesting loss of structural integrity. In conclusion, both artichoke leaf and
stem extracts exerted concentration-dependent effects on dermal fibroblasts. While the leaf
extract demonstrated stronger proliferative and wound-healing activity at optimal
concentrations, the stem extract was revealed with limited efficacy and cytotoxicity at higher
doses. These findings suggest that artichoke-derived compounds, particularly from the leaf,
may have therapeutic potential in promoting skin regeneration, whose phytochemical analysis
is in progress in our laboratory.