Effect of vitamin D on free radical metabolism in renal tissues of septic rats Septik ratların böbrek dokularında serbest radikal metabolizmasına vitamin D’nin etkisi


Atikeler G., Çıracı M. Z. , Kocabıyık M., KAVUTÇU M., Canbolat O., ÇEVİK C.

Turkish Journal of Biochemistry, vol.41, no.5, pp.316-321, 2016 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 41 Issue: 5
  • Publication Date: 2016
  • Doi Number: 10.1515/tjb-2016-0046
  • Title of Journal : Turkish Journal of Biochemistry
  • Page Numbers: pp.316-321
  • Keywords: Vitamin D, Sepsis, Renal toxicity, Antioxidant enzymes, OXIDATIVE PARAMETERS, SEPSIS, ANTIOXIDANT, EPIDEMIOLOGY, SUPEROXIDE, ENZYMES, STRESS, INJURY

Abstract

© 2016, Turkish Biochemistry Society. All rights reserved.Objective: Sepsis is a common cause of morbidity and mortality in the intensive care unit. Lipopolysaccharide (LPS)-induced excessive immune response is associated with multi-organ damage in sepsis. Excessive immune response causes multi-organ damage by increasing oxidative stress. Studies on the antioxidant effects of vitamin D demonstrated its protective role. In this study we aimed to investigate the effects of vitamin D on free radical metabolism in LPS injected rats. Methods: Twenty four wistar albino rats were separated into control, sepsis, sepsis+vitamin D and vitamin D groups. Sepsis was induced with single injection of LPS Esherichia coli (O111-b4) 16 mg/kg. Vitamin D was given 2 mg/kg 25 (OH) single dose via gavage for 3 days. Renal function tests were analyzed in serum. Tissue catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and glutathione-S-transferase (GST) activities were analyzed, and rat renal tissues were evaluated histopathologically. Results: SOD and GSH-Px activities were not significantly different between the groups. CAT activities were significantly decreased in all groups compared to control, this suppression was seen in the sepsis+vitamin D group versus sepsis group. GST activities were significantly decreased in sepsis and sepsis+vitamin D group compared to control, but GST activities were significantly elevated vitamin D group compare to sepsis and sepsis+vitamin D group. Blood urea nitrogen (BUN) and creatinine levels were significantly elevated in sepsis and sepsis+vitamin D group. Inflammation, expansion in bowman capsule were detected in sepsis and sepsis+vitamin D groups. Conclusion: Vitamin D treatment does not seem to have protective role against renal toxicity in sepsis.Nutrition with vitamin D in sepsis may have suppressive effect on antioxidant enzymes sush as CAT and GST due to reduced substrat level which use hydrogen peroxide (H2O2) and GSH as a substrate.