Effect of vitamin D on free radical metabolism in renal tissues of septic rats Septik ratların böbrek dokularında serbest radikal metabolizmasına vitamin D’nin etkisi


Atikeler G., Çıracı M. Z., Kocabıyık M., KAVUTÇU M., Canbolat O., ÇEVİK C.

Turkish Journal of Biochemistry, vol.41, no.5, pp.316-321, 2016 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 41 Issue: 5
  • Publication Date: 2016
  • Doi Number: 10.1515/tjb-2016-0046
  • Journal Name: Turkish Journal of Biochemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.316-321
  • Keywords: Vitamin D, Sepsis, Renal toxicity, Antioxidant enzymes, OXIDATIVE PARAMETERS, SEPSIS, ANTIOXIDANT, EPIDEMIOLOGY, SUPEROXIDE, ENZYMES, STRESS, INJURY
  • Lokman Hekim University Affiliated: Yes

Abstract

© 2016, Turkish Biochemistry Society. All rights reserved.Objective: Sepsis is a common cause of morbidity and mortality in the intensive care unit. Lipopolysaccharide (LPS)-induced excessive immune response is associated with multi-organ damage in sepsis. Excessive immune response causes multi-organ damage by increasing oxidative stress. Studies on the antioxidant effects of vitamin D demonstrated its protective role. In this study we aimed to investigate the effects of vitamin D on free radical metabolism in LPS injected rats. Methods: Twenty four wistar albino rats were separated into control, sepsis, sepsis+vitamin D and vitamin D groups. Sepsis was induced with single injection of LPS Esherichia coli (O111-b4) 16 mg/kg. Vitamin D was given 2 mg/kg 25 (OH) single dose via gavage for 3 days. Renal function tests were analyzed in serum. Tissue catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and glutathione-S-transferase (GST) activities were analyzed, and rat renal tissues were evaluated histopathologically. Results: SOD and GSH-Px activities were not significantly different between the groups. CAT activities were significantly decreased in all groups compared to control, this suppression was seen in the sepsis+vitamin D group versus sepsis group. GST activities were significantly decreased in sepsis and sepsis+vitamin D group compared to control, but GST activities were significantly elevated vitamin D group compare to sepsis and sepsis+vitamin D group. Blood urea nitrogen (BUN) and creatinine levels were significantly elevated in sepsis and sepsis+vitamin D group. Inflammation, expansion in bowman capsule were detected in sepsis and sepsis+vitamin D groups. Conclusion: Vitamin D treatment does not seem to have protective role against renal toxicity in sepsis.Nutrition with vitamin D in sepsis may have suppressive effect on antioxidant enzymes sush as CAT and GST due to reduced substrat level which use hydrogen peroxide (H2O2) and GSH as a substrate.