Gastroprotective effect of orexin-A and heme oxygenase system


Gemici B., Tan R. , BİRSEN İ., Izgüt Uysal V. N.

Journal of Surgical Research, vol.193, no.2, pp.626-633, 2015 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 193 Issue: 2
  • Publication Date: 2015
  • Doi Number: 10.1016/j.jss.2014.08.048
  • Title of Journal : Journal of Surgical Research
  • Page Numbers: pp.626-633
  • Keywords: Orexin-A, Ischemia-reperfusion, Gastric, Lesion, Heme oxygenase-1, Heme oxygenase-2, INDUCED GASTRIC DAMAGE, VASCULAR ENDOTHELIAL-CELLS, NITRIC-OXIDE SYNTHASE, ISCHEMIA-REPERFUSION, OXIDATIVE STRESS, RATS, APOPTOSIS, PATHWAY, INJURY, CYTOPROTECTION

Abstract

© 2015 Elsevier Inc. All rights reserved.Background Orexin-A, besides playing an important role in the mechanism of food intake, exhibits a potent gastroprotective action against the formation of acute gastric mucosal injury. The aim of the present study was to determine the effect of administered orexin-A against ischemia-reperfusion (I/R)-induced gastric injury on the expression of heme oxygenase (HO)-1 and HO-2 in gastric tissue.Materials and methods Wistar rats were subjected to 30 min of ischemia followed by 3 h reperfusion. Orexin-A was infused at a dose of 500 pmol/kg/min during the I/R period. The lesion area was measured by stereomicroscope. The myeloperoxidase activity and 4-hydroxinonenol-malondialdehyde content of gastric mucosa were evaluated spectrophotometrically, and the gastric tumor necrosis factor-α was measured by enzyme linked immune sorbent assay. The expression of HO-1 and HO-2 was determined by Western blotting analysis.Results Orexin-A significantly decreased the I/R-induced gastric lesions, myeloperoxidase activity, and 4-hydroxinonenol-malondialdehyde concentration in gastric tissue exposed to I/R. The gastroprotective effect of orexin-A in gastric I/R model was accompanied by the increase in HO-2 expression and the decrease in HO-1 expression.Conclusions Orexin-A exerts a protective action on gastric mucosa subjected to I/R, and this effect is associated with the reduction of neutrophil infiltration and lipid peroxidation in gastric tissue in addition to the increase in HO-2 expression due to the administration of orexin-A.