© 2020, Romanian Society for Pharmaceutical Sciences. All rights reserved.Different habitats of seas and oceans make these ecosystems enormous sources of bioactive natural compounds. Marine species like terrestrial species produce terpenes, steroids, fatty acids, etc. In this study, we describe the isolation and structure identification of three fatty acids ethyl esters, 9-pentadecenoic acid ethyl ester, 9-heptadecenoic acid ethyl ester and 5,8,11,14,17-docosapentaenoic acid ethyl ester from Anemonia viridis coast of Bodrum (Turkish coast). The structure identification was made by NMR and Mass spectroscopic methods. Furthermore, the antioxidant and cytotoxic activity of extract were determined using the superoxide radical scavenging method and the MTT assay, respectively. Acetylcholinesterase inhibition and tyrosinase inhibition activities of methanolic extract of A. viridis were also screened. As a result, A. viridis methanolic extract possesses dose-dependent cytotoxic activity, moderate superoxide radical scavenging and tyrosinase inhibition activity. However, it was inactive against acetylcholinesterase enzyme. This is the first study on isolation of secondary metabolites and bioactivity screening of A. viridis from Turkey.