Assessment of peripheral DNA damage by alkaline comet assay in maintenance hemodialysis subjects with hepatitis C infection


Horoz M., Bolukbas C., Bolukbas F. F., KOÇYİĞİT A., Aslan M., Koylu A. O., ...More

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, vol.596, no.1-2 SPEC. ISS., pp.137-142, 2006 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 596 Issue: 1-2 SPEC. ISS.
  • Publication Date: 2006
  • Doi Number: 10.1016/j.mrfmmm.2005.12.009
  • Journal Name: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.137-142
  • Keywords: end-stage renal disease, hemodialysis, hepatitis C, comet assay, DNA damage, GEL-ELECTROPHORESIS ASSAY, CHRONIC DIALYSIS PATIENTS, STAGE RENAL-DISEASE, UREMIC PATIENTS, HEPATOCELLULAR-CARCINOMA, OXIDATIVE DAMAGE, LIVER-DISEASE, RISK-FACTORS, VIRUS, STRESS
  • Lokman Hekim University Affiliated: Yes

Abstract

Despite the high prevalence of hepatitis C infection among hemodialysis subjects, there is no information concerning the DNA damage of hepatitis C (+) hemodialysis subjects. We aimed to find out if there is any additional effect of hepatitis C infection on peripheral DNA damage in maintenance hemodialysis subjects. Fifteen hepatitis C (+) and 22 hepatitis C (-) hemodialysis subjects, 21 hepatitis C subjects without renal disease, and 22 healthy controls were enrolled. Peripheral DNA damage was assayed using alkaline comet assay. Median DNA damage levels of the study groups were as follows: hepatitis C (+) maintenance hemodialysis subjects, 88 (0-232); hepatitis C (-) maintenance hemodialysis subjects, 58 (0-228); hepatitis C (+) subjects without renal disease, 112 (44-252); controls, 26 (0-72). DNA damage level was significantly higher among hepatitis C (+) subjects without renal disease than hepatitis C (-) maintenance hemodialysis subjects and healthy controls (both p < 0.05/6). Both maintenance hemodialysis subjects with and without HCV infection had significantly higher DNA damage level than healthy controls (both p < 0.05/6). DNA damage level was comparable between hepatitis C (+) subjects without renal disease and HCV (+) hemodialysis subjects, and between hemodialysis subjects with and without hepatitis C infection (all p > 0.05/6). Linear regression analysis revealed that hepatitis C infection was the only independent factor in predicting the peripheral DNA damage (p < 0.05, β = 0.395). Each one of end-stage renal disease and hepatitis C infection significantly increases DNA damage level. However, in hemodialysis subjects, hepatitis C infection does not cause significant additional increase in DNA damage level, and it may be partly due to protective effect of hemodialysis on hepatitis C infection. © 2006 Elsevier B.V. All rights reserved.