Does moxifloxacin alter oxidant status in the cornea? An experimental study


Akal A., Ulas T., Goncu T., Guldur M. E. , Kocarslan S., Taskin A., ...More

Cutaneous and Ocular Toxicology, vol.34, no.2, pp.139-143, 2015 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 34 Issue: 2
  • Publication Date: 2015
  • Doi Number: 10.3109/15569527.2014.918138
  • Title of Journal : Cutaneous and Ocular Toxicology
  • Page Numbers: pp.139-143
  • Keywords: Apoptosis, cornea, intracameral moxifloxacin, oxidative stress, PROPHYLACTIC INTRACAMERAL MOXIFLOXACIN, 0.5-PERCENT OPHTHALMIC SOLUTION, STRESS-MEDIATED APOPTOSIS, IN-VITRO SAFETY, OXIDATIVE STRESS, CATARACT-SURGERY, GATIFLOXACIN 0.3-PERCENT, ENDOPHTHALMITIS, TOXICITY, LEVOFLOXACIN

Abstract

© 2014 Informa Healthcare USA, Inc.Objective: In this experimental study, we investigated the possible effects of intracameral moxifloxacin on oxidative stress parameters and endothelial cell morphology in corneal tissue. Methods: In total, 30 rats were randomly assigned to three groups of 10 rats: the sham group (Group 1, n=10); the control group (Group 2), where balanced salt solution (BSS) was administered at a dose of 0.01 cc (n=10); and the treatment group (Group 3), where moxifloxacin was administered at a dose of 0.05 mg/0.01 cc (n=10). Total antioxidant status (TAS) and total oxidant status (TOS) in corneal tissue and blood samples were measured and the oxidative stress index (OSI) was calculated. Also, corneal tissue histopathology was evaluated with caspase-3 and caspase-8 staining. Apoptotic activity was also evaluated. Results: In blood samples, TAS, TOS, and OSI levels were not statistically significantly different (all p>0.05). Compared with the sham and control groups, TOS and OSI levels in cornea tissue were significantly different in the moxifloxacin group (all p<0.05). However, compared with the control group, no statistically significant difference was found in the sham group (all p>0.05). Compared with the sham and control groups, apoptotic activity was higher in the moxifloxacin group, in both immunohistochemical staining for caspase-3 and caspase-8. Conclusions: Intracameral moxifloxacin injection seems to be safe systemically, but it may have toxic effects on corneal tissues, as suggested by oxidative stress parameters and a histopathological evaluation.