Neuroprotective Effects of Hesperidin and Naringin in SK-N-AS Cell as an In Vitro Model for Alzheimer’s Disease

Kuşi M., Becer E., Vatansever H. S., YÜCECAN S.

Journal of the American Nutrition Association, vol.42, no.4, pp.418-426, 2023 (Scopus) identifier identifier


Objective: Hesperidin and naringin are flavonoids that are found in citrus fruits. Our aim was to create an in vitro model of Alzheimer’s disease (AD) and to evaluate the neuroprotective effects of hesperidin and naringin in SK-N-AS and AD model cells. Methods: Aβ25-35 was used to create an AD model in SK-N-AS cells. The cytotoxicity of hesperidin and naringin was evaluated using MTT. β-amyloid, tau and α-synuclein distributions were analyzed using indirect immunoperoxidase staining to investigate the neuroprotective effects of hesperidin and naringin. Results: The AD model was created by 1 µM of Aβ25-35 for 48 hours after ThT staining. The intensity of β-amyloid was reduced through both hesperidin and naringin treatment in AD model cells. Both flavonoids significantly decreased the intensity of α-synuclein in SK-N-AS and AD model cells. Conclusions: Hesperidin and naringin can be potentially used as neuroprotective agents. Naringin may be more effective than hesperidin in the accumulation of β-amyloid and tau proteins.