Preparation and characterization of bFGF and BSA-loaded microspheres

ÇETİN M., ÇAPAN Y., Vural I., Dogan A., Guc D., Hincal A., ...Daha Fazla

JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY, cilt.15, sa.5, ss.371-375, 2005 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 15 Sayı: 5
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1016/s1773-2247(05)50067-4
  • Dergi Adı: JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.371-375
  • Anahtar Kelimeler: PLGA, microspheres, BSA, bFGF, SDS-PAGE, FIBROBLAST-GROWTH-FACTOR, BLOOD-BRAIN-BARRIER, POLY(LACTIDE-CO-GLYCOLIDE) MICROSPHERES, DELIVERY-SYSTEM, RELEASE, PROTEIN, NANOPARTICLES, NANOSPHERES, GLYCOLIDE, ISCHEMIA
  • Lokman Hekim Üniversitesi Adresli: Hayır

Özet

This article describes the preparation and characterization of BSA and bFGF-loaded PLGA microspheres and assesses the effect of entrapment procedure and in vitro release conditions. In the experiments, changing the PVA concentration from 3 to 5% (w/v) in the pre-emulsion innerphase led to a change in particle size and encapsulation efficiency of BSA-loaded and bFGF-loaded PLGA microspheres from 1 to 0.7 mu m and from 73 to 65%, respectively. The in vitro release of the bFGF from PLGA microspheres was much lower than BSA-loaded PLGA microspheres. For all of the formulations, BSA and bFGF retained integrity after the microencapsulation process as evaluated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Besides, the cell proliferation-stimulating activity of bFGF was evaluated by using BALB/c 3T3 cells and it was shown that the bioactivity of bFGF was increased by adding heparin to the release medium.