Akademik Veri Yönetim Sistemi
33rd International Symposium on Pharmaceutical and Biomedical Analysis, Ankara, Türkiye, 2 - 06 Temmuz 2023, ss.218, (Özet Bildiri)
Octreotide is a synthetic octapeptide derivative that has pharmacological effects similar to natural somatostatin but with a much longer duration of action. Octreotide is widely utilized in clinical practice for patients with neuroendocrine cancers. In the last decade, an increasing incidence of neuroendocrine cancers has been reported. In this current study, a new rapid method for the analysis of octreotide from plasma by LC/MS-MS was developed. The analyses of octreotide and lanreotide (internal standard) were performed in positive ionization. The separations were performed on a C18 analytical column with a gradient mobile phase consisting of a mixture of 0.1% formic acid in water and 0.1% formic acid in acetonitrile at a flow rate of 0.3 mL/min. The total run time was 5 min. The injection volume was 10 μL and the column temperature was maintained at 55°C. Plasma sample preparation was based on organic solvent precipitation of proteins. The method was linear in the range of 1-100 ng/ mL for octreotide in plasma. Finally, the plasma samples from cancer patients treated with octreotide were quantified with the developed LC-MS/MS method.