Quartz crystal microbalance biosensor for label free MDA MB 231 cancer cell detection via Notch-4 receptor
TALANTA, vol.204, no.1, pp.840-845, 2019 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 204 Issue: 1
- Publication Date: 2019
- Doi Number: 10.1016/j.talanta.2019.06.060
- Journal Name: TALANTA
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.840-845
- Lokman Hekim University Affiliated: No
Abstract
Breastcanceristhemostcommoncancerinwomenwithincreasinginsidance.Breastcanceroccursasaresultof somemolecularchanges, suchasaberrantordysregulatedexpressionof receptors, inbreast epithelial cells. Therefore,breastcancercellscanbedetectedthroughtheirmembranereceptorsusingspecificantibodies.This studyaimstoformaquartzcrystalmicrobalance(QCM)biosensortodetectbreastcancercells.Notchreceptor signalingdirectsmanypathways indevelopingbreast tissueanditsexpressionisfoundtobeaberrantordisregulatedinmetastaticbreastcancercells.Itsinvolvementinmalignanttransformationmakesitapotentialdrug target. Thehumanmetastaticbreast cancer cells,MDAMB231cells, areusedhereasamodel due to the overexpressionofnotch-4receptorontheirmembranes.First, toincreasethesurfaceareaof thechippoly(2hydroxyethylmethacrylate-PHEMA)nanoparticleswere synthesizedandwereplacedonQCMchipsurface. Then, thesurfacewas furthermodifiedandfunctionalizedbybindingnotch-4receptorantibodyusingcarbodiimide.NanoparticlecoatedandantibodyattachedQCMchipswerecharacterizedviaFTIR-ATR,ellipsometry, contactanglemeasurementsandbyatomicforcemicroscopy.MDAMB231cell samplesranginginnumbers between50-300cells/mlwereintroducedtothefunctionalizedQCMchipataflowrateof1.0mL/minandthe resonancefrequency(f0)wasrecorded.Then,cellsampleswereappliedtotheQCMbiosensorandtheresonance frequencywasmonitored.ThebindingmodefittedbesttoLangmuirisothermmodel.Sensitivityisfoundtobe highandtheselectivityastestedbycompetitiveadsorptionofL929mousefibroblastcellsshowedthatQCM biosensorwas17.5timesmoreselectiveforMDAMB231cellsthanthefibroblastcells.Thechipwasreusable andwasstableover3months.Theseresultsindicatethat, thenotch-4receptorantibodyPHEMAnanoparticle QCMbiosensorishighlyselectiveandefficientsystemthatmaybeusedforcancercelldetection.