Quartz crystal microbalance biosensor for label free MDA MB 231 cancer cell detection via Notch-4 receptor

Özden A. K.

TALANTA, cilt.204, sa.1, ss.840-845, 2019 (SCI-Expanded)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 204 Sayı: 1
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1016/j.talanta.2019.06.060
  • Dergi Adı: TALANTA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.840-845
  • Lokman Hekim Üniversitesi Adresli: Hayır

Özet

Breastcanceristhemostcommoncancerinwomenwithincreasinginsidance.Breastcanceroccursasaresultof somemolecularchanges, suchasaberrantordysregulatedexpressionof receptors, inbreast epithelial cells. Therefore,breastcancercellscanbedetectedthroughtheirmembranereceptorsusingspecificantibodies.This studyaimstoformaquartzcrystalmicrobalance(QCM)biosensortodetectbreastcancercells.Notchreceptor signalingdirectsmanypathways indevelopingbreast tissueanditsexpressionisfoundtobeaberrantordisregulatedinmetastaticbreastcancercells.Itsinvolvementinmalignanttransformationmakesitapotentialdrug target. Thehumanmetastaticbreast cancer cells,MDAMB231cells, areusedhereasamodel due to the overexpressionofnotch-4receptorontheirmembranes.First, toincreasethesurfaceareaof thechippoly(2hydroxyethylmethacrylate-PHEMA)nanoparticleswere synthesizedandwereplacedonQCMchipsurface. Then, thesurfacewas furthermodifiedandfunctionalizedbybindingnotch-4receptorantibodyusingcarbodiimide.NanoparticlecoatedandantibodyattachedQCMchipswerecharacterizedviaFTIR-ATR,ellipsometry, contactanglemeasurementsandbyatomicforcemicroscopy.MDAMB231cell samplesranginginnumbers between50-300cells/mlwereintroducedtothefunctionalizedQCMchipataflowrateof1.0mL/minandthe resonancefrequency(f0)wasrecorded.Then,cellsampleswereappliedtotheQCMbiosensorandtheresonance frequencywasmonitored.ThebindingmodefittedbesttoLangmuirisothermmodel.Sensitivityisfoundtobe highandtheselectivityastestedbycompetitiveadsorptionofL929mousefibroblastcellsshowedthatQCM biosensorwas17.5timesmoreselectiveforMDAMB231cellsthanthefibroblastcells.Thechipwasreusable andwasstableover3months.Theseresultsindicatethat, thenotch-4receptorantibodyPHEMAnanoparticle QCMbiosensorishighlyselectiveandefficientsystemthatmaybeusedforcancercelldetection.