Akademik Veri Yönetim Sistemi
7th International Eurasian Conference on Biological and Chemical Sciences, Ankara, Türkiye, 2 - 04 Ekim 2024, ss.112
Mesenchymal stem cells(MSCs) secrete exosomes
that influence important mechanisms of cancer cells, such as proliferation,
apoptosis, metastasis, angiogenesis, chemoresistance, and
epithelial-mesenchymal transition. The effects of MSC derived exosomes on tumor
cells and their potential for modification and use in cancer therapy are being
investigated. In this study, the aim was to clarify the effects of Umbilical Cord(UC)
derived MSCs on the apoptosis and cell cycle mechanisms of pancreatic
adenocarcinoma cells. In this study, after particle size, number, and marker
analyses were performed, the UC-MSCs exosomes were commercially obtained and treated
to Panc-1 cells at 4000, 8000, and 12000 exosomes per cell for 24 hours.
Apoptosis analysis was performed using Annexin V staining, and cell cycle
analysis was performed using Propidium Iodide staining, both analyzed by flow
cytometry. In the cell cycle analysis, the percentage of cells in the S phase
was found to be 31.87% in the non-treated group, 29.98% in the 1:4000 group,
28.4% in the 1:8000 group, and 27.68% in the 1:12000 group. In the apoptosis
analysis, the percentage of total apoptotic cells was 12.07% in the non-treated
group, 13.04% in the 1:4000 group, 13.24% in the 1:8000 group, and 13.15% in
the 1:12000 group. Our preliminary findings suggest that UC-MSC derived
exosomes do not induce proliferation of Panc-1 cells. We also demonstrated that
they do not significantly affect apoptosis of Panc-1 cells. Further explanation
of the effects of UC-MSC derived exosomes on other antitumor molecular
mechanisms will be an important step in the development of potential
therapeutic approaches.