Enhancing the Regenerative Potential of Adipose-Derived Mesenchymal Stem Cells through TLR4-Mediated Signaling

Kaçaroğlu D., Yaylacı S.

CURRENT STEM CELL RESEARCH AND THERAPY, vol.19, pp.1-20, 2024 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 19
  • Publication Date: 2024
  • Doi Number: 10.2174/011574888x283664231219080535
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Biotechnology Research Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE
  • Page Numbers: pp.1-20
  • Lokman Hekim University Affiliated: Yes


Toll-like receptor 4(TLR4) is a receptor that traditionally plays an important role in

immunomodulation (regulation of the immune system) and the initiation of proinflammatory

responses. TLR4 is used in the body to recognize molecular patterns of pathogens or damaged cells

from outside. However, in recent years it has also become clear that TLR4 can affect not only the

immune system, but also the function of stem cells, especially mesenchymal stem cells. Therefore,

understanding how TLR4 signaling works at the cellular and molecular level and using this knowledge

in regenerative medicine could be potentially useful, especially in the treatment of adipose-derived

mesenchymal stem cells (ADMSCs). How these cells can use TLR4 signaling when used to increase

their regenerative potential and repair tissues is an area of research. This study aims to elucidate the

multifaceted role of TLR4-mediated signaling in ADMSCs. Employing a comprehensive set of assays,

including MTT for cell viability, flow cytometry for surface marker expression, and gene expression

analysis, we demonstrate that TLR4 activation significantly modulates key aspects of ADMSC biology.

Specifically, TLR4 signaling was found to regulate ADMSCs proliferation, surface marker expression,

and regenerative capacity in a dose- and time-dependent manner. Furthermore, TLR4 activation

conferred cytoprotective effects against Doxorubicin (DOX)-induced cellular apoptosis. These

findings suggest that TLR4 signaling could be used to enhance the regenerative abilities of ADMSCs

and enable ADMSC-based therapies to be used more effectively for tissue engineering and

therapeutic purposes. However, it is important to note that research in this area needs more details

and clinical studies.