Akademik Veri Yönetim Sistemi
16thInternational Symposium on Health Informatics andBioinformatics, Ankara, Türkiye, 4 - 06 Ekim 2023, ss.10, (Özet Bildiri)
mpact of antibiotic prophylaxis and delivery mode on the newborn microbial composition is still poorly understood. The aim of this study is to determine the amount of prophylactic antibiotics given to mothers in cesarean section (CS) in breast milk and to examine the possible effect on newborn meconium microbiota. According to the study protocol, first-pass postpartum meconium specimens were collected from 21 newborns and BM specimens were collected from 21 mothers on the 7th postnatal day (CS=12, vaginal delivery (VD=9). CS received a single dose of ampicillinsulbactam 2 g before surgical incision; CS and VD received cefuroxime 1 g treatment according to the hospital’s prophylaxis protocol. For meconium microbiome analysis, 16S rRNA V3-V4 regionspecific amplicon sequence was performed on Illumina NovaSeq 6000 platform, raw sequence data and microbial population analysis was performed with QIIME 2 (version 2022.11). Antibiotic breast milk was quantified with liquid chromatography-mass spectroscopy (LC/MS). Our work was supported by TUBITAK with the project number 222S764 within the scope of the 1002-Quick Support-B projects and also by the Health Sciences University Scientific Research Coordinator with the project number 2022-023While 7 of the meconium samples did not have any reads, 14 meconium samples (7 CS, 7 VD), in which sufficient reads sequence data were obtained, were evaluated. There was no significant difference between CS and VD groups in terms of alpha diversity in meconium samples (observed_features, shannnon). At the phylum level, the most abundant taxonomic groups were Actinobacteriota, Firmicutes, Bacteroidota, Proteobacteria, Acidobacteriota in all samples. Actinobacteria were found to be the most abundant phylum in both groups (Mec-VD, 89.63%; Mec-CS, 94.49%). The second dominant phylum, Firmicutes, was observed at lower rates (CS 2.64%, VD 6.09%) in meconium samples. At genus level, Bifidobacterium was the most dominant genus in all groups. According to LefSe analysis, significantly abundant taxa in VD groups were Propionibacteriales, Propionibacteriaceae and Cutibacterium (synonymous Propionibacterium). Cefuroxime was detected in all breast milk samples. The mean amount of cefuroxime was similar in CS and VD groups (CS, 52.92±28.08; and VD, 40.21±15.83; p=0.572).