Background: Sensitivity complaints are commonly observed in teeth affected by MIH (molar incisor hypomineralization). Aim: This study aimed to evaluate the hypersensitivity observed in MIH-affected teeth and the effect of desensitizing agents applied with and without ozone to incisors affected by MIH. Study Design: The first part of the study included 120 teeth from 42 patients with MIH. These 42 patients included 33 children with 92 incisor teeth with a Vas score of ≥30, and these 92 incisors were included in the second part of the study. The patients included in the second part were divided into three main groups and six subgroups. The main groups included the following: fluoride, CPP-ACP and CPP-ACP with fluoride. Each main group was divided into two subgroups: one with ozone use and one without ozone use. Results: Girls exhibited significantly more sensitivity compared with boys (p<0.05). There were significant decreases in hypersensitivity compared to baseline in all of the groups (p<0.05). There were no differences among the groups at the end of the study (p0.05). Conclusion: The results of this study revealed that gender is an important factor in the sensitivity of teeth with MIH. Desensitizing agents effectively reduced the hypersensitivity of teeth with MIH. CPP-ACP paste was found to be more effective, and ozone therapy prolonged the effect of CPP-ACP paste.