Akademik Veri Yönetim Sistemi
Ionics, 2026 (SCI-Expanded, Scopus)
6-Benzylaminopurine (6-BAP), is a synthetic cytokinin extensively used in plant tissue culture and agricultural biotechnology. It has recently attracted increasing concern due to its potential biological and genotoxic effects on animal and human cells. Hence, the development of an environmentally friendly, rapid, and reliable analytical methods for its determination in complex matrices is of great importance. In this study, we developed a rapid, green, and low-cost electrochemical approach for the determination of 6-BAP in apple juice. An electrochemically reduced graphene oxide-modified glassy carbon electrode (ErGO/GCE) was fabricated via a simple electrodeposition process, significantly enhancing analytical sensitivity. Differential pulse voltammetry (DPV) measurements exhibited a linear response in the concentration range of 2.00 × 10⁻⁷ to 8.00 × 10⁻⁶ M, with a limit of detection 2.94 × 10⁻⁸ M. The sensor exhibited excellent reproducibility (relative standard deviation of 2.44%) and high recovery (101.74%) in real sample analysis. Furthermore, a double-stranded DNA (dsDNA)-based electrochemical biosensor was constructed to explore the molecular interaction between 6-BAP and DNA, providing valuable insights into its possible genotoxic effects. In the range of 2.00 × 10− 7 and 2.00 × 10− 6 M, the greatest decrease in the peak currents of dGuo and dAdo was observed at an interaction concentration of 6 × 10⁻⁷ M 6-BAP, with an optimal interaction time of 180 s. In a bulk incubated solution, the dAdo signals also dropped by around 100.0% to 1.6%. This study offers an environmentally sustainable and cost-effective electrochemical approach with strong potential for routine monitoring of 6-BAP and for evaluating its biological impacts in food safety.