We aimed to study the effect of Diabetes Mellitus type 2 on CETP activity and to evaluate the relationship of CETP activity levels with Chol, TG, and HDL. Blood was drawn in the morning after an overnight fast from 136 subjects (70 Diabetic obese patients, 66 obese subjects as control group) CETP activity were measured in Serum using Fluorescence assay, whereas TC,TG, HDL and Glu were measured using Enzymatic colorimetric assay. There was no significant difference between CETP activity in diabetic obese patients and obese subjects (60.38, 58.15 pmol/μl/1hr, respectively; P=0.28). There was considering direct relation for CETP activity with BMI, TC, and TG (P=0.03, P<0.0001, P= 0.001, respectively) while this activity correlated negatively with HDL-c in Diabetic obese patients group. This study showed a considering relation between serum CETP levels with TC and HDL-c in diabetic patients and obese groups. This suggests that CETP may affect the dyslipidemia noted in type 2 diabetes particularly obese one.