Simultaneous spectrophotometric determination of mefenamic acid and paracetamol in a pharmaceutical preparation using ratio spectra derivative spectrophotometry and chemometric methods


DİNÇ E., Yücesoy C., ONUR F.

Journal of Pharmaceutical and Biomedical Analysis, vol.28, no.6, pp.1091-1100, 2002 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 28 Issue: 6
  • Publication Date: 2002
  • Doi Number: 10.1016/s0731-7085(02)00031-6
  • Journal Name: Journal of Pharmaceutical and Biomedical Analysis
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1091-1100
  • Keywords: mefenamic acid, paracetamol, ratio spectra derivative method, chemometric methods, pharmaceutical preparation, LEAST-SQUARES, PSEUDOEPHEDRINE HYDROCHLORIDE, MULTIVARIATE CALIBRATION, MULTICOMPONENT ANALYSIS, QUANTITATIVE-ANALYSIS, PREDICTION METHODS, PYRIDOXINE HCL, THIAMINE HCL, FORMULATIONS, RESOLUTION
  • Lokman Hekim University Affiliated: Yes

Abstract

Four new methods are described for the simultaneous determination of mefenamic acid (MEF) and paracetamol (PAR) in their combination. In the first method, ratio spectra derivative method, analytical signals were measured at the wavelengths corresponding to either maximums or minimums for both drugs in the first derivative spectra of the ratio spectra obtained by dividing the standard spectrum of one of two drugs in 0.1 M NaOH:methanol (1:9). In the chemometric techniques, classical least-squares, inverse least-squares and principal component regression (PCR), the training was randomly prepared by using the different mixture compositions containing two drugs in 0.1 M NaOH:methanol (1:9). The absorbance data was obtained by the measurements at 13 points in the wavelength range 235-355 nm in the absorption spectra. Chemometric calibrations were constructed by the absorbance data and training set for the prediction of the amount of MEF and PAR in samples. In the third chemometric method, PCR, the covariance matrix corresponding to the absorbance data was calculated for the basis vectors and matrix containing the new coordinates. The obtained calibration was used to determine the title drugs in their mixture. Linearity range in all the methods was found to be 2-10 μg/ml of MEF and 4-20 μg/ml of PAR. Mean recoveries were found satisfactory (>99%). The procedures do not require any separation step. These methods were successfully applied to a pharmaceutical formulation, tablet, and the results were compared with each other. © 2002 Elsevier Science B.V. All rights reserved.