Akademik Veri Yönetim Sistemi
CHEMISTRY AND BIODIVERSITY, cilt.23, sa.4, ss.1-24, 2026 (SCI-Expanded, Scopus)
Macrochloa tenacissima (Stipa tenacissima) is an economically important species, but its biochemical properties remainunderexploited. This study reports the phenolic composition of M. tenacissima ethanolic extract (MTEE) and evaluates itsantimicrobial virulence potential. Total phenolic (7.42 ± 0.46 mg GAE/g) and flavonoid (6.49 ± 0.36 mg QE/g) contentswere determined. LC-ESI-MS/MS revealed quinic acid (5.699 mg/g), p-coumaric acid (0.639 mg/g), protocatechuic acid(0.473 mg/g), protocatechuic aldehyde (0.319 mg/g), and vanillin (0.354 mg/g) as the most abundant phenolics. Minimal inhibitoryconcentration (MIC) against Staphylococcus aureus, Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Candida albicans,and Chromobacterium violaceum indicated S. aureus and C. albicans as the most susceptible, with MICs of 0.312 mg/mL. TheMTEE inhibited violacein production by C. violaceum CV12472, ranging from 50.06% ± 0.55% (MIC) to 9.06% ± 0.89% (MIC/4),and antiquorum sensing activity (7.0 ± 0.0 mm) against C. violaceum CV026 at MIC. Antibiofilm activity at MIC was 81.23% ± 1.15%(S. aureus), 80.91% ± 2.37% (C. albicans), 75.50% ± 2.68% (E. coli), and 71.84% ± 1.43% (S. typhi) and decreased in a concentration-dependent manner. MTEE inhibited swarming and swimming motilities against P. aeruginosa. Molecular docking experimentsindicated favorable interactions, and the negative binding energies substantiate the in vitro antimicrobial virulence effects of M.tenacissima. Further, molecular dynamics simulations (MDs) have been carried out to investigate the stability of compounds inthe binding of the chosen targets. The ADMET study has been performed to estimate the drug-likeness and the toxicity of theMTEE components.© 2026 Wiley-VHCA AG.Chemistry & Biodiversity, 2026; 23:e03675https://doi.org/10.1002/cbdv.2025036751 of 24