Introduction: Nonsteroidal anti-inflammatory drugs (NSAIDs), which inhibit prostaglandin synthesis, are used in many clinical areas. Although they are advantageous because of their analgesic, anti-inflammatory, and antipyretic properties, their effects on tissue healing are controversial. Dexketoprofen and lornoxicam are widely used, new-generation NSAIDs. However, their effects on fibroblasts, which are important for healing, are unknown. Herein, we aimed to investigate the effects of these two drugs on fibroblast viability, migration, and collagen expression.

Materials and Methods: L929 mouse fibroblasts were cultured in vitro. Viability assays, wound-healing assays, and gene expression analyses were performed after 24 hours of treatment with lornoxicam and dexketoprofen at a 0–1-mM dosage. The viability test, wound-healing test, and gene expression analysis were performed using the MTT method, by calculating the closed area, and by qRT–PCR, respectively.

Results: Lornoxicam and dexketoprofen at doses of 0–1 mM dose-dependently decreased viability, and changes in cell morphology were observed. Lornoxicam 1 µM and dexketoprofen 10 µM doses significantly reduced the migration rate of L929 cells compared with the control group (****p < 0.0001). After 24 hours, COL1A1 expression in L929 cells was 0.027 and 0.015 when 1 and 10 µM of lornoxicam and dexketoprofen were applied.

Discussion and Conclusion: Lornoxicam and dexketoprofen have negative effects on the viability, migration, and type 1 collagen synthesis of fibroblasts. Considering these negative effects on fibroblasts is crucial while using these drugs.