In this preliminary study, a public survey was planned to investigate frequency of chronic fatigue syndrome (CFS) among nurses employed in primary and secondary health care centers and its relationship with sociodemographic factors and education. The poll designed by Family Medicine Department of Dicle University Medical Faculty involved 14 open-ended and multiple-choice questions about demographic features work conditions habits and hobbies. The criteria of Center for Disease Control (CDC) for defining chronic fatigue syndrome were also added into the poll. In April 2004, 33 nurses from Diyarbakir State Hospital, Diyarbakir Children Hospital, Diyarbakir Chest Disease Hospital and Batikent Primary Health Care Clinic participated the survey. Two academicians from Dicle University Atatürk Health Institute worked as polestars to complete the survey face-to-face interviews with each nurse. Mean age of the nurses was 29.2±7.9 years. There were four cases (4/33, 12.1%) matching the criteria of CDC for CFS and none of them were found to be related with a chronic disease state. A total of 10 cases (10/33 30.3%) were grouped with diagnosis of CFS, chronic idiopathic fatigue (CIF) and prolonged fatigue (PF). Number of cases with CIF were three (3/33, 9.1%) and one nurse was found to have PF (1/33, 3.0 %). Among 24 cases without CFS. two had some of additional findings. Nurses have CFS, CIF and PF due to stressed work shift and other sources of stress. However, it was observed that marital status and education level did not occur any effect on chronic fatigue.