Akademik Veri Yönetim Sistemi
9th International Congress of Molecular Medicine, 18 - 20 Aralık 2023, ss.1
Mesenchymal stem cells(MSCs) are morphologically similar to fibroblasts with high regenerative capacity, can differentiate into cells of mesodermal origin. Due to different therapeutic properties, MSCs are clinically used in haematological malignancies, cardiovascular diseases, neurological diseases, organ transplants, endocrine diseases and regenerative medicine. However, the regenerative properties and stem cell potency of ADMSCs cannot be adequately preserved by conventional cell culture techniques. To solve this problem, HAVDI pentapeptide sequence, which is located in the first extracellular part of N-cadherin and is involved in cell-cell interactions, was used. Although the environment increases MSC proliferation and chondrogenic differentiation, its effects on regeneration and stem cell potency have not been sufficiently investigated. In this study, experimental groups were formed using plastic surface, HAVDI and scrambled HAVDI sequences and cultured in 2D and 3D environment. ADMSCs were cultured for 48h and collected for PCR and Flow cytometry analysis. A fold increase in MMP2;13.2, VEGFR2;24.5 and PLAU;32.2 genes was observed in 3D HAVDI experimental group compared to 2D plastic surface.In the 2D HAVDI experimental group, an increase in the expression of CD90+8.7%, CD73+7.4%, CD105+8.1% surface markers was observed compared to 2D plastic surface. In the 3D HAVDI experimental group, increased expression of CD90+5.5%, CD73+8%, CD105+3.3% surface markers was observed compared to 2D. The results showed a significant increase in the expression of regenerative genes and stem cell markers in cell cultures with HAVDI peptide amphiphile. In conclusion, HAVDI peptide sequence can be considered as a strategy to increase the effectiveness of MSCs commonly used in clinics.