Akademik Veri Yönetim Sistemi
FARMACIA, cilt.69, sa.5, ss.960-966, 2021 (SCI-Expanded)
The objectives of this study were to analyse the presence of two aryltetralin group lignans (podophyllotoxin and picropodophyllotoxin), in order to evaluate the total phenolic and the flavone contents and to determine the biological activities of Linum arboreum. Aryltetralin-lignans are major compounds of Linum genus. In this study, a fast, simple, accurate and selective HPLC method was performed for the qualitative and quantitative analysis of podophyllotoxin and picropodophyllotoxin, by using reverse-phase high performance liquid chromatography (HPLC) with diode array detector (DAD). The HPLC analysis was performed on different fractions, obtained by using methanol, ethyl acetate, water, hexane and chloroform as extraction solvents, from both seeds and aerial parts. The identification of podophyllotoxin and picropodophyllotoxin is based on the retention time and the UV spectra by comparison with standards. According to the International Conference on Harmonization (ICH) guidelines, the method was evaluated, and precision, accuracy, and reproducibility of the method have been apparent from good linearity. Podophyllotoxin and picropodophyllotoxin were found in the aerial parts of L. arboreum L. in higher amounts than seeds Furthermore, total phenolic and total flavonoid contents and antioxidant, cytotoxicity and antimicrobial activity of L. arboreum were investigated. While the fractions presented dose dependent antioxidant activity, they exhibited weak antimicrobial activity. The seeds ethyl acetate fraction presented the highest cytotoxic activity against HCT-116 cell line. The fractions have shown dose dependent antioxidant activity.