Akademik Veri Yönetim Sistemi
International Journal of Morphology, cilt.43, sa.1, ss.116-122, 2025 (SCI-Expanded)
It is known that mediators including prostaglandins, nitric oxide (NO), and hydrogen sulfide (H2 S) have a role in contraction and relaxation in a variety of tissues, including the bladder. This study aimed to evaluate the contribution of nitrergic and prostaglandinergic pathways to the possible effect of H2 S and the interaction between these pathways on bladder. The study was performed on male Wistar rat’s bladder strips. The effects of NaHS and L-cysteine on carbachol-mediated or EFS-evoked contractile responses were measured. The responses were also measured with cyclooxygenase (COX) inhibitors incubation and NOS inhibitor incubation. Additionally, COX inhibitors and L-NAME were incubated in combination in EFS-mediated responses of NaHS. The highest concentrations of NaHS and L-cysteine caused inhibition on carbachol-mediated responses. No statistical significance was detected in the responses in COX inhibitors (FBP, ASA, NFA) or L-NAME incubations. Upon EFS-evoked contractile responses, when NaHS was applied, it caused inhibition at the highest concentration. FBP and NFA significantly increased the effect of NaHS. While L-cysteine alone did not have a relaxant effect but statistically significant inhibition with ASA and FBP incubation. COX inhibitors and L-NAME co-incubation reversed NaHS effect at only certain concentrations. Our study shows that nitric oxide, prostaglandins, and hydrogen sulfide are mediators that interact in the contractile or relaxation function of the bladder. However, how this interaction occurs has not yet been clearly revealed. With further studies on this subject, it seems possible that they can become a new treatment target in bladder dysfunction.