A completely human-derived biomaterial mimicking limbal niche: Platelet-rich fibrin gel

Dereli Can G., Akdere Ö. E., Can M. E., AYDIN B., ÇAĞIL N., GÜMÜŞDERELİOĞLU M.

Experimental Eye Research, vol.173, pp.1-12, 2018 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 173
  • Publication Date: 2018
  • Doi Number: 10.1016/j.exer.2018.04.013
  • Journal Name: Experimental Eye Research
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1-12
  • Keywords: Limbal epithelial stem cell deficiency, Limbal explant culture, Human-derived platelet-rich fibrin, Human-derived amniotic membrane, Xenobiotic-free cell culture, Chemical-free cell culture, EX-VIVO EXPANSION, EPITHELIAL STEM-CELLS, OCULAR SURFACE, AMNIOTIC MEMBRANE, CLINICAL-OUTCOMES, GROWTH-FACTORS, FEEDER CELLS, TRANSPLANTATION, RELEASE, CULTURE
  • Lokman Hekim University Affiliated: No


© 2018 Elsevier LtdPlatelet-rich fibrin (PRF) is a natural biomaterial and has excellent biochemical and physical properties with a history of proven biocompatibility in the field of tissue engineering and regenerative medicine. Recent reports of fibrin-based matrices have offered new opportunities to apply PRF as a supplement for in vitro cell culture. Here, custom-modified human-derived PRF (HPRF) was produced via different centrifugation protocols, then, characterized by morphologically and chemically and utilized as a substrate and as a conditioned medium for limbal explant culture for the first time. It was found that the HPRF released significantly higher levels of growth factors which are essential for epithelial cell growth. The enhanced physicochemical properties of the HPRF were also proven in the limbal explant cultures in terms of cell growth, migration, viability, and stemness in comparison with the conventional limbal explant culture on human-derived amniotic membrane. Consequently, HPRF hydrogels are appealing natural biomaterials for the purpose of mimicking limbal niche and the discovery elucidates this new, xeno-chemical-free, completely human-derived biomaterial can be utilized as a supplement to promote epithelial cell behaviour in vitro.