An investigation on uropathogen Escherichia coli strains with regard to antimicrobial susceptibility and extended spectrum beta- lactamase


Fabad Journal of Pharmaceutical Sciences, vol.34, no.4, pp.173-177, 2009 (Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 34 Issue: 4
  • Publication Date: 2009
  • Journal Name: Fabad Journal of Pharmaceutical Sciences
  • Journal Indexes: Scopus
  • Page Numbers: pp.173-177
  • Keywords: Antimicrobial susceptibility, E.coli, ESBL
  • Lokman Hekim University Affiliated: No


Urinary tract infections (UTI) are among the most commonly encountered infections and are thus associated with widespread usage of antibiotics. Escherichia coli is the commonest bacteria isolated in UTI and treatment alternatives are limited in extended spectrum beta-lactamase (ESBL) producers. The aim of this study was to determine antibiotic susceptibility, ESBL production of the Escherichia coli strains and comparison of (ESBL) (+) and ESBL (-) strains, those were isolated in the Clinical Microbiology Laboratory, from urine samples of patients who presented to Ankara Dişkapi Yildirim Beyazit Training Hospital with urinary tract infections, in a period between January 2007 and June 2007. Conventional bacteriologic methods were used in identification and a total of 130 E.coli species were isolated. Sensitivity tests were performed according to the guidelines and criteria of National Committee for Clinical Laboratory Standards (NCCLS) using Kirby-Bauer disk diffusion method. The highest susceptibility rates were detected in amikacin(95%) and cefoperazone- sulbactam (91%). ESBL production was investigated by double disk synergy method and 27 (21%) of the isolated E.coli strains were found to be ESBL producers. Gentamicin and piperacillin-tazobactam susceptibility in ESBL (-) strains were found higher than ESBL (+) strains. An increase in the rate of resistance to ciprofloxacine (96%) in ESBL (+) strains was noted as well.