Isolation of the lignan 6-methoxypodophyllotoxin from Linum boissieri and biosynthesis of lignans in this species


KONUKLUGİL B., Fuss E., Alfermann W.

Pharmaceutical Biology, vol.43, no.9, pp.737-739, 2005 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 43 Issue: 9
  • Publication Date: 2005
  • Doi Number: 10.1080/13880200500406313
  • Journal Name: Pharmaceutical Biology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.737-739
  • Keywords: lignan, Linum boissieri, 6-methoxypodophyllotoxin, CELL, PODOPHYLLOTOXIN, CULTURES
  • Lokman Hekim University Affiliated: No

Abstract

We were able to establish a suspension culture of Linum boissieri that produces 6-methoxypodophyllotoxin (6MPT). As a first step to gain insight into the lignan biosynthesis in L. boissieri cell cultures, we were able to measure phenylalanine ammonia-lyase (PAL) activity in raw protein extracts. PAL is a key enzyme in the early part of the general phenylpropanoid pathway, leading (beside others) to the precursors for lignan biosynthesis. © 2005 Taylor & Francis Ltd.