Effect of melatonin on cerebral edema in rats


Görgülü A., Palaoǧlu S., Ismailoǧlu Ö., Tuncel M., Sürücü M. T., Erbil M., ...Daha Fazla

Neurosurgery, cilt.49, sa.6, ss.1434-1442, 2001 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 49 Sayı: 6
  • Basım Tarihi: 2001
  • Doi Numarası: 10.1097/00006123-200112000-00024
  • Dergi Adı: Neurosurgery
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1434-1442
  • Anahtar Kelimeler: Cold injury, Edema, Melatonin
  • Lokman Hekim Üniversitesi Adresli: Hayır

Özet

OBJECTIVE: Melatonin (5-methoxy-N-acetyltrypamine), a chemical naturally produced in the pineal gland, has been suggested to be a free radical scavenger and an antioxidant. In the present study, the effect of melatonin on cold-induced brain edema was evaluated by determination of cerebral water content, blood-brain barrier permeability, and areas of infarct; the effects were also studied histopathologically. METHODS: Brain edema was produced in rats by creating a lesion via cortical freezing. Animals were separated into four groups: sham-operated (craniectomy only); control (cold injury); sham-vehicle (cold injury plus saline); and melatonin treatment (cold injury plus melatonin). Melatonin was administered (50 mg/kg intraperitoneally) 15 minutes after the cold injury was induced. Twenty-four hours later, tissue samples from the core, from the periphery of the cold-injured hemisphere, and from the contralateral hemisphere symmetrical to the cold injury were obtained. RESULTS: Melatonin treatment reduced edema (mean ± standard deviation; 86.22 ± 1.54% in the control group versus 80.78 ± 2.76% in the melatonin treatment group, P < 0.001) and blood-brain barrier permeability (45.34 ± 2.75% in the control group versus 38.26 ± 3.40% in the melatonin treatment group, P < 0.001) at the periphery of cold injury. Area of infarct reduced from 5.84 ± 0.58% in the control group to 3.30 ± 0.89% in the melatonin treatment group (P < 0.001). The effect of melatonin was also confirmed histopathologically. CONCLUSION: Melatonin was found to be neuroprotective in instances of cold-induced brain edema. Thus, melatonin may be a valuable therapeutic agent in the treatment of cerebral edema.