Anticholinesterase and antioxidant effects of the ethanol extract, ethanol fractions and isolated flavonoids from Cistus laurifolius L. leaves

AKKOL E., ERDOĞAN ORHAN İ., YEŞİLADA E.

FOOD CHEMISTRY, cilt.131, sa.2, ss.626-631, 2012 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 131 Sayı: 2
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1016/j.foodchem.2011.09.041
  • Dergi Adı: FOOD CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.626-631
  • Anahtar Kelimeler: Cistus laurifolius, Cholinesterase inhibition, Antioxidant, Ethanol extract, Flavonoid, AGLYCONES, TURKEY, MICE
  • Lokman Hekim Üniversitesi Adresli: Hayır

Özet

In the current study, the n-hexane, chloroform, ethyl acetate, water, and n-butanol fractions obtained from the main ethanol extract of Cistus launfolius L were evaluated for their cholinesterase inhibitory effects against acetyl- (AChE) and butyrylcholinesterase (BChE), at 50, 100, and 200 mu g/ml, using an ELISA microplate reader. The antioxidative effect of the extract and fractions was also determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, ferrous ion-chelation capacity, and ferric-reducing antioxidant power (FRAP) test systems. Total phenol and flavonoid contents of the extract and fractions were calculated using Folin-Ciocalteau and aluminium chloride reagents. Three flavonoid derivatives; 3-O-methylquercetin (1), 3,7-O-dimethylquercetin (2), and 3,7-O-dimethylkaempferol (3) isolated from the CHCl(3) fraction were also tested in the same manner. Our experimental findings indicated that the ethanol extract exerted the highest AChE inhibition (80.07 +/- 1.06% at 200 mu g/ml). The ethyl acetate and n-butanol fractions displayed the best activity against DPPH and FRAP assays. (C) 2011 Elsevier Ltd. All rights reserved.