Antifungal and cytotoxic activity of withanolides from acnistus arborescens

Roumy V., Biabiany M., Hennebelle T., Aliouat E. M., Pottier M., Joseph H., ...Daha Fazla

Journal of Natural Products, cilt.73, sa.7, ss.1313-1317, 2010 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 73 Sayı: 7
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1021/np100201p
  • Dergi Adı: Journal of Natural Products
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1313-1317
  • Lokman Hekim Üniversitesi Adresli: Hayır

Özet

Three compounds were isolated from Acnistus arborescens, a tree commonly used in South and Central America in traditional medicine against several infectious diseases, some of which are caused by fungi. Bioassay-guided fractionation of a MeOH extract of leaves, based on its anti-Pneumocystis carinii activity, led to the isolation of compounds 1-3. Mono- and bidimensional NMR analyses enabled identification of two new withanolides, (20R,22R)-5β,6β-epoxy-4β,12β,20-trihydroxy-1-oxowith-2-en- 24-enolide (1) and (20R,22R)-16β-acetoxy-3β,4β;5β,6β- diepoxy-12β,20-dihydroxy-1-oxowith-24-enolide (2), and withanolide D (3). Antifungal activity on 13 fungi responsible for human infections (five dermatophytes, one nondermatophyte mold, six yeasts, and Pneumocystis carinii) was examined. Cytotoxicity of these compounds was also evaluated in vitro. © 2010 The American Chemical Society and American Society of Pharmacognosy.