Antifungal and cytotoxic activity of withanolides from acnistus arborescens

Roumy V., Biabiany M., Hennebelle T., Aliouat E. M., Pottier M., Joseph H., ...More

Journal of Natural Products, vol.73, no.7, pp.1313-1317, 2010 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 73 Issue: 7
  • Publication Date: 2010
  • Doi Number: 10.1021/np100201p
  • Journal Name: Journal of Natural Products
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1313-1317
  • Lokman Hekim University Affiliated: No

Abstract

Three compounds were isolated from Acnistus arborescens, a tree commonly used in South and Central America in traditional medicine against several infectious diseases, some of which are caused by fungi. Bioassay-guided fractionation of a MeOH extract of leaves, based on its anti-Pneumocystis carinii activity, led to the isolation of compounds 1-3. Mono- and bidimensional NMR analyses enabled identification of two new withanolides, (20R,22R)-5β,6β-epoxy-4β,12β,20-trihydroxy-1-oxowith-2-en- 24-enolide (1) and (20R,22R)-16β-acetoxy-3β,4β;5β,6β- diepoxy-12β,20-dihydroxy-1-oxowith-24-enolide (2), and withanolide D (3). Antifungal activity on 13 fungi responsible for human infections (five dermatophytes, one nondermatophyte mold, six yeasts, and Pneumocystis carinii) was examined. Cytotoxicity of these compounds was also evaluated in vitro. © 2010 The American Chemical Society and American Society of Pharmacognosy.