Clearing and embedding in polyester resin for demonstrating the nerve distribution pattern of skeletal muscles

PEKER T. V., GÜLEKON İ. N., Anil A., Turgut H., Liman F.

Biotechnic and Histochemistry, cilt.78, sa.3-4, ss.187-190, 2003 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 78 Sayı: 3-4
  • Basım Tarihi: 2003
  • Doi Numarası: 10.1080/10520790310001594026
  • Dergi Adı: Biotechnic and Histochemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.187-190
  • Anahtar Kelimeler: Embedding, Innervation pattern, Polyester, Sihler's stain, Skeletal muscle
  • Lokman Hekim Üniversitesi Adresli: Hayır

Özet

The preservation of many stained gross specimens in solution creates some difficulties. It is convenient and effective to preserve material in polyester resin instead of glycerol. The aim of this study was to determine the usefulness of clearing and embedding using polyester resin. The samples consisted of the nerve distribution patterns of skeletal muscles stained using Sihler's method. The muscles were cleared more successfully and the intramuscular nerve distributions were demonstrated better in polyester than in glycerol. The method presented here eliminates not only the storage and handling problems of specimens, but also problems such as pale stains and the molding of preparations. Furthermore, it is more convenient to examine and to photograph specimens cleared and embedded in polyester than those stored in glycerol.