Akademik Veri Yönetim Sistemi
Lokman Hekim Health Sciences , cilt.2, sa.2, ss.60-64, 2022 (Hakemli Dergi)
Background and Aims: Metastatic breast cancer causes high mortality in women. Therefore, the search for effective treatment continues. The suitable radiation dose should be determined in order to cause minimum damage to the surrounding tissue. We aimed to demonstrate if cell culture systems can be used to demonstrate the effects of different radiation doses over breast cancer cells. Methods: Here, MDA-MB-231 breast cancer cells were used. The antiproliferative effect of different doses of X-rays was measured by MTT test. Wound healing test was performed to assess the metastatic potential and flow cytometry analysis was used to evaluate the cell cycle. Results: It is found that, 2Gy, 4Gy and 8Gy radiation doses were applied to the cells. And cell proliferation was significantly decreased at all doses(p<0,05). However, there was not a significant decrease between the different dose groups(p>0.05). The lateral mobilization potential of MDA-MB-231 cells was decreased significantly in radiation applied cells as compared to the control. Cell cycle analyzes showed that different doses of radiation delayed the cells in G1 as compared to control cells in the S phase. Conclusion: In the cell culture system, a decrease in proliferation and metastatic potential of breast cancer cells by the application of different doses of radiation is found. Therefore, for effective planning of radiation therapy additional parameters of radiation should also be assessed via cell culture systems. It has been shown that cell culture systems may be used for dosimetry studies of radiation therapy of breast cancer. Keywords: Breast cancer, Molecular Biology, Cell Biology, Cell culture, Molecular Biology, Radiotherapy