Laser irradiation has been used in clinical and experimental studies in recent years. However, some effects produced at the cellular level have not been explained adequately. In order to give more information regarding some positive effects of laser radiation on various biological systems, levels of muscle Cu, Zn, Mg, Ca, Cd and K were measured in irradiated and control rats. After irradiation with a near-IR (infrared) laser light (IR/CEB-S Space Laser Company, wavelength 904 nm, peak power 5 W, pulse durations 100-150 ns, power density 0.6 mW/cm2, divergence 70 mrad) 10 min daily for ten days, irradiated and control rats were killed by cervical dislocation. In the irradiated group the levels of Cu, Mg, Ca, Cd of skeletal muscle decreased with respect to the control group significantly while level of Zn increased (p < 0.05); but there was no significant difference between the levels of K in these two groups.