Electrochemical sensor for the analysis of 5-hydroxymethylcytosine in the presence of cytosine using pencil graphite electrode

BİLGE YÜCEL S., Gürbüz M. M., ÖZKAN S. A., DOĞAN TOPAL B.

Analytical Biochemistry, vol.696, 2025 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 696
  • Publication Date: 2025
  • Doi Number: 10.1016/j.ab.2024.115674
  • Journal Name: Analytical Biochemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Compendex, EMBASE, Veterinary Science Database
  • Keywords: 5-Hydroxymethylcytosine, Pencil graphite electrode, Square wave voltammetry, Stripping voltammetry
  • Lokman Hekim University Affiliated: No

Abstract

In recent years, important efforts have been made to elucidate the mechanisms of epigenetic regulation, and one of the most studied epigenetic modifications was DNA methylation/demethylation. In this study, the voltammetric behaviour of 5-hydroxymethylcytosine was studied in the pH range of 2.00–11.00 using pencil graphite electrodes by differential pulse and square wave voltammetry. The effect of buffer solutions, scan rate, square wave voltammetry parameters, and stripping conditions on the voltammetric responses of 5-hydroxymethylcytosine were performed. The electrochemical oxidation process of 5-hydroxymethylcytosine on the pencil graphite electrode was realized under adsorption control. In human urine, by square wave stripping voltammetry, 5-hydroxymethylcytosine was quantified in a concentration range of 1.00 × 10−5 M-2.00 × 10−4 M. The proposed method was tested in the presence of cytosine in human urine. The recovery value of 5-hydroxymethylcytosine was found to be 99.57 %.