Quercetin Differentially Modulates MTT-Derived Cellular Metabolic Activity and Apoptosis-Related Gene Expression in A549 and BEAS-2B Cells
METABOLITES, cilt.16, sa.10, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 16 Sayı: 10
- Basım Tarihi: 2026
- Doi Numarası: 10.3390/metabo16100740
- Dergi Adı: METABOLITES
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Chemical Abstracts Core, EMBASE, Directory of Open Access Journals, Natural Science Collection (ProQuest), Biological Science Database (ProQuest)
- Lokman Hekim Üniversitesi Adresli: Evet
Özet
Background/Objectives: Quercetin is a dietary flavonoid with potential anticancer activity, but comparative effects in malignant and non-malignant lung-derived epithelial cells remain incompletely characterized. Methods: A549 and BEAS-2B cells were exposed to quercetin (1-250 & micro;M) for 24, 48, and 72 h, and MTT-derived cellular metabolic activity was measured. Mean normalized concentration-response curves from three independent biological experiments (n = 3) were fitted with four-parameter logistic models, and Greenhouse-Geisser-corrected cell line & times; concentration & times; time interactions were tested. Apoptosis-related gene expression was assessed by qRT-PCR after exposure to 45 & micro;M quercetin for 48 h; 45 & micro;M was not a discrete concentration in the MTT series and was close to the directly tested 40 & micro;M condition. Results: Apparent MTT-derived IC50 values were 58.28 & micro;M (95% CI 44.18-74.81) for A549 and 121.22 & micro;M (89.50-164.66) for BEAS-2B at 48 h, and 2.73 & micro;M (2.31-3.67) and 1.49 & micro;M (1.27-1.77), respectively, at 72 h. Cell line & times; concentration interactions were significant at all exposure times, as was the cell line & times; concentration & times; time interaction; transcriptional profiles differed descriptively between cell lines. Conclusions: The fitted IC50 ordering reversed between 48 and 72 h, so a stable cancer-selective response is not supported. MTT reflects metabolic activity rather than cell death, and transcript changes do not establish functional apoptosis.