Akademik Veri Yönetim Sistemi
Anestezi Dergisi, cilt.12, sa.2, ss.135-138, 2004 (Scopus)
Tramadol is a centrally acting synthetic analgesic with opioid, serotonergic and noradrenergic mechanisms of action. We aimed to investigate the interaction of nitric oxide synthase (NOS) inhibitors L-NAME and 7 Nitro indazole (7NI) with tramadol by using a chemical pain model. Seventy two adult female Swiss Webster mice were randomly allocated into 3 groups to receive either intraperitoneal saline, L-NAME 10 mg kg-1 or 7-NI 3 mg kg-1. Each main group was divided into 4 subgroups to make 12 groups. 20 minutes after the first injection, another intraperitoneal injection of saline, Tramadol 10 mg kg-1, Tramadol 20 mg kg-1 or Tramadol 40 mg kg-1 were applied to the subgroups of these three main groups. The mice in all groups received 3% acetic acid 300 mg kg-1 i.p. another 20 minutes after the second injections. Writhing of mice were observed following 5 minutes after injections, for the subsequent 15 minutes. No statistically significant differences were observed with the three doses of Tramadol or saline when the pretreatments with saline, L-NAME or 7-NI were compared. In 7-NI pretreated group, 10, 20, 40 mg kg-1 Tramadol subgroups were similar, with the exception of saline subgroup (p<0.05). Tramadol 20 and 40 mg kg-1 subgroups of Saline and L-NAME groups, a significant decrease was found in writhing numbers when compared to saline and Tramadol 10 mg kg-1 subgroups (p<0.05). We concluded that 20 mg kg-1 tramadol injection provided enough analgesic effect and the nitric oxide synthase inhibitors L-NAME or 7NI did not significantly influence the analgesic effect of tramadol in the chemical pain model we used.