Akademik Veri Yönetim Sistemi
33rd International Symposium on Pharmaceutical and Biomedical Analysis, Ankara, Türkiye, 2 - 06 Temmuz 2023, ss.122
A simple, accurate and sensitive spectrophotometric method for
quantitative analysis of Emtricitabine (ETB) and Tenofovir Disoproxil Fumarate
(TDF) in pharmaceutical dosage forms has been developed. Ratio spectra first
derivative spectrophotometry was used for this purpose. In the method; first
derivative spectra of the ratio spectra of the solutions of ETB and TDF in
distilled water were recorded between 200 and 350 nm. 10 μg/mL solution of ETB
and 10 μg/mL solution of ETB were used as divisors. First derivative spectra
were recorded as ∆λ= 4 nm. Derivative absorbance values were read at 222 and
242 nm in their derivative spectra. Mean recovery and relative standard
deviation of the method were found as % 99.2 – 101.2 and % 0,8 - 1,1
respectively in the method. Working range of the method was found as 2-15 μg/mL
for both ETB and TDF with an excellent linearity (r² = 0,999). Developed method
was successfully applied to tablet formulation containing ETB/TDF and
commercially available in Turkiye. According to the results we have found that
this method can be applied for the routine simultaneous analysis of ETB and TDF
in pharmaceutical formulations.
Keywords: Tenofovir Disoproxil Fumarate, Emtricitabine,
Spectrophotometric, Ratio, First Derivative