Preparation and characterization of poly(D,L-lactide-co-glycolide) microspheres for controlled release of human growth hormone


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ÇAPAN Y., Jiang G., Giovagnoli S., Na K., DeLuca P. P.

AAPS PharmSciTech, cilt.4, sa.2, 2003 (Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 4 Sayı: 2
  • Basım Tarihi: 2003
  • Doi Numarası: 10.1208/pt040228
  • Dergi Adı: AAPS PharmSciTech
  • Derginin Tarandığı İndeksler: Scopus
  • Anahtar Kelimeler: Composite microspheres, Human growth hormone, Microspheres, Protein delivery
  • Lokman Hekim Üniversitesi Adresli: Hayır

Özet

The purpose of this research was to assess the physicochemical properties of a controlled release formulation of recombinant human growth hormone (rHGH) encapsulated in poly(D,L-lactide-co-glycolide) (PLGA) composite microspheres. rHGH was loaded in poly(acryloyl hydroxyethyl) starch (acHES) microparticles, and then the protein-containing microparticles were encapsulated in the PLGA matrix by a solvent extraction/evaporation method. rHGH-loaded PLGA microspheres were also prepared using mannitol without the starch hydrogel microparticle microspheres for comparison. The detection of secondary structure changes in protein was investigated by using a Fourier Transfer Infrared (FTIR) technique. The composite microspheres were spherical in shape (44.6 ± 2.47 μm), and the PLGA-mannitol microspheres were 39.7 ± 2.50 μm. Drug-loading efficiency varied from 93.2% to 104%. The composite microspheres showed higher overall drug release than the PLGA/mannitol microspheres. FTIR analyses indicated good stability and structural integrity of HGH localized in the microspheres. The PLGA-acHES composite microsphere system could be useful for the controlled delivery of protein drugs.