An experimental design approach to selecting the optimum LC conditions for the determination of local anaesthetics

Dincel A., BAŞCI AKDUMAN N. E.

Chromatographia, cilt.66, sa.SUPPL. 1, 2007 (SCI-Expanded, Scopus)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 66 Sayı: SUPPL. 1
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1365/s10337-007-0275-x
  • Dergi Adı: Chromatographia
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: column liquid chromatography, experimental design, method optimization and validation, local anaesthetics, SOLID-PHASE EXTRACTION, AQUEOUS-HUMOR, GAS-CHROMATOGRAPHY, MASS-SPECTROMETRY, CATARACT-SURGERY, HUMAN SERUM, BUPIVACAINE, VALIDATION, PROPARACAINE, PLASMA
  • Lokman Hekim Üniversitesi Adresli: Hayır

Özet

A sensitive high performance liquid chromatographic (HPLC) method has been developed and validated for the simultaneous determination of four local anaesthetics: lidocaine, proparacaine, bupivacaine and oxybuprocaine. A full factorial design was used. The chromatographic separation was achieved using a Bondesil C8 (4.6 × 2.5 mm i.d., particle size 5 μm) analytical column. An optimised mobile phase consisted of acetonitrile and sodium dihydrogen phosphate (pH = 3.0, 20 mM) (30:70, v/v) at a flow rate of 1.2 mL min-1. Local anaesthetics detection was performed by UV-Vis detector at 220 nm. The retention times for lidocaine, proparacaine, bupivacaine and oxybuprocaine were 5.74, 9.28, 16.84 and 26.26 min, respectively. HPLC-UV-Vis method was linear in the range of 50-5,000 ng mL-1 for lidocaine and proparacaine and 100-5,000 ng mL-1 for bupivacaine and oxybuprocaine. The limit of detection (LOD) was 25 ng mL-1 for lidocaine, proparacaine and 30 ng mL-1 for bupivacaine and oxybuprocaine. The limit of quantification (LOQ) was found to be 50 ng mL -1 for lidocaine, proparacaine and 100 ng mL-1 for bupivacaine, oxybuprocaine. In intra-day and inter-day precision and accuracy analysis, the relative standard deviation was found to be less than 8%. © 2007 Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH.